피봉수 Bong Soo Pi , 최동원 Dong Won Choi , 박성일 Sung-il Park , 남진 Jin Nam , 김연준 Youn Joon Kim , 한상훈 Sang Hoon Han

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Abstract

Recently, the number of customers who want natural cosmetics without petroleum originated cosmetic ingredients is increasing over the world, however, there are few natural cosmetics that claim functionality in the products. Because there are few functional cosmetic ingredients from nature and though so, it is very difficult to stabilize the final products manufactured by using those functional cosmetic ingredients in the long period. This study is on stabilization of oleanolic acid, insoluble anti-wrinkle cosmetic ingredients from nature, with hydrogenated lecithin in the solubilization product, aiming for developing a formulation of solubilization products which can be certified as organic cosmetics. In case of other synthetic solubilizers, they showed unstability at 45 ℃ within 2 ～ 3 days, however, for natural origin hydrogenated lecithin a stable product was made without particle size change during 4 weeks. The stability of oleanolic acid in the solubilization product was measured for 24 weeks at 25 ℃ and 40 ℃, both samples showed over 90 wt% value of the initial one, which can prove that oleanolic acid was stabilized in the product without any degradation. Also through clinical tests, the effect of moisturizing, anti-wrinkle, safety were confirmed.

Key Words

hydrogenated lecithin, oleanolic acid, stability, solubilization, anti-wrinkle

김경남 Kyung Nam Kim , 전상훈 Sang Hoon Jeon , 심민경 Min Kyung Shim , 최경호 Kyung Ho Choi , 최영진 Yeong Jin Choi , 한상훈 Sang Hoon Han

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Abstract

The skin condition after dermatological surgery can be changed. As the laser light penetrates into the skin during the procedure it will activate biomolecules which are the cause of the related redness. In addition, it can parch sensitive skin and worsen itching and flaking. The original intention of ‘BB cream’ is for soothing sensory skin, but not for make-up. The commonly used ‘BB cream’ is suitable for all skin types and are targeted specially to those who have sensitive and acne-prone skin. It has the advantages of both skin-care and make-up as it improves the skin condition and evens out the skin tone while providing coverage for redness spots and other blemishes. Our study focuses to give the make-up function that naturally correct the brightness and contrast of redness skin, to realize the skin-care function that is effective against sensitive skin.

Key Words

face make up, BB cream, dermatological surgery, erythema

김지혜 Ji Hye Kim , 김민정 Min Jung Kim , 최수기 Su Ki Choi , 배승희 Seung Hee Bae , 안성관 Sung Kwan An , 윤영민 Yeong Min Yoon

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Abstract

Lemon and eucalyptus oils were known to have various biological effects such as anti-aging and sterilizing action. However these essential oils were not well studied about their antioxidant activity and antimicrobial activity against specific skin flora. In this study, we investigated antioxidant activity and antimicrobial activity of lemon and eucalyptus oils by using DPPH radical scavening activity and paper disc method. Lemon oil showed the high DPPH scavenging activity, while eucalyptus oil did not. Blending oils of lemon and eucalyptus had little enhancememt on antioxidant activity. Paper disc method demonstrated that each oil had a high antimicrobial effect against C. albicans and P. acnes in a concentration dependent manner. The blending oils had enhancememt on antimicrobial effect against P. acnes. In conclusion, the blending oil of lemon and eucalyptus can be used as a more effective natural agents for cure of skin trouble and acne.

Key Words

lemon essential oil, eucalyptus essential oil, antimicrobial, antioxidant, skin flora

김아름 A Reum Kim , 김정은 Jung Eun Kim , 박수남 Soo Nam Park

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Abstract

In this study, the antioxidative effect, antibacterial, inhibitory effects on tyrosinase, inhibitory effects on elastase and component analysis of Phellinus linteus (P. linteus) extracts were investigated. The ethyl acetate fraction of P. linteus extracts (2.94 μg/mL) showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC₅₀). Reactive oxygen species (ROS) scavenging activity (OSC₅₀) of P. linteus extracts on ROS generated in Fe³⁺-EDTA/H₂O₂ system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction (0.0072 μg/mL) showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of P. linteus extracts on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The P. linteus extracts showed cellular membrane protective effects in a concentration dependent manner (5 ~ 50 μg/mL). The inhibitory effect (IC₅₀) on tyrosinase of P. linteus extract was the highest at 50 % ethanol extract (6.34 μg/mL), and the inhibitory effect (IC₅₀) on elastase of P. linteus was the highest at ethyl acetate fraction (14.08 μg/mL). TLC, HPLC chromatogram and LC/ESI-MS of the ethyl acetate fraction obtained from P. linteus extracts were identified interfungin A (PL RPT-1a). These results indicate that extract/fractions of P. linteus can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging ROS, and protect cellular membranes against ROS. Extract/fractions of P. linteus can be applicable to new cosmeceuticals for antioxidant, antiaging, antiwrinkle and whitening.

Key Words

Phellinus linteus, antioxidative activity, antiaging activity, LC/ESI-MS, component analysis

이수연 Su Yeon Lee , 이새미 Sae Mi Lee , 허우범 Woo Beom Heo , 김진국 Jin Guk Kim , 김영희 Young Heui Kim

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Abstract

In order to investigate the potential of Nigella sativa (N. sativa) oil as an active ingredient for whitening cosmetics, we prepared N. sativa oil. We measured its inhibitory effects on mushroom tyrosinase activity, cellular ty-rosinase activity, and melanin synthesis inhibitory activity in B16 melanoma cells. N. sativa oil and its components showed inhibitory activity against mushroom tyrosinase and melanin synthesis. In a melanin synthesis inhibition assay using mouse B16-F10 melanoma cell, it reduced melanin production up to 86 % at a concentration of 10 mg/mL without cytotoxicity. In the study on the melanogenic protein expressions by using RT-PCR and Western blot, N. sativa oil and its components inhibited expression of tyrosinase protein, which is a well-known key protein on melano-genesis, and tyrosinase expression was gradually decreased in a dose-dependent manner. Therefore, this result sug-gests that N. sativa oil could be used as an active ingredient for whitening cosmetics.

Key Words

skin-whitening, Nigella sativa, thymoquinone, thymohydroquinone, B16 melanoma

진무현 Mu Hyun Jin , 정의택 Eui Taek Jeong , 김미선 Mi-sun Kim , 송혜진 Hye Jin Song , 곽택종 Taek-jong Kwak , 박선규 Sun Gyoo Park , 이상민 Sang Min Lee

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Abstract

5,4'-dihydroxystilbene-3-O-β-D-glucopyranoside (Polydatin) is one of the stilbenes found in Polygonum cuspidatum (P. cuspidatum), however, the effects of polydatin on skin biology remain to be elucidated. In this study, we obtained polydatin from P. cuspidatum and investigated the effects of polydatin in skin-derived melanocytes and fibroblasts. In melanocytes, polydatin inhibited not only the tyrosinase activity and melanin production but the expression of melanogenic factors, tyrosinase and microphthalmia-associated transcription factor (MITF). In addition, the level of type I procollagen in fibroblasts was analyzed, and polydatin significantly induced the production of type I procollagen in a dose-dependent manner. Finally we conformed that topical treatment of polydatin improved wrinkle and induced whitening of human skin in vivo. These data provide evidence that polydatin can be a potent candidate for the improvement of both skin wrinkle and whitening from the point of industry view.

Key Words

Polygonum cuspidatum, 5, 4'-dihydroxystilbene-3-O-D-glucopyranoside, melanogenesis, MITF, collagen synthesis

유동찬 Dong-chan Yoo , 조현남 Hyun-nam Cho , 김경란 Kyoung-ran Kim , 변혜정 Hae-jung Byun , 김정현 Jung-hyun Kim , 박혜빈 Hye-bin Park , 김희준 Hee-joon Kim , 방대석 Dae-suk Bang , 양선아 Seun-ah Yang , 강공원 Gong-won Khang , 정호순 Ho-soon Jeong , 지광환 Kwang-hwan Jhee

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Abstract

In this report, we have investigated the functional properties change of phytochemicals by the encapsula-tion using water soluble host, β-cyclodextrin. The cream, shampoo, bodywash, and hair tonic containing phytochem-ical supramolecules were produced by mixing the surfactants, fragrances and the oriental herbal extracts encapsulated with β-cyclodextrin. Shampoo and bodywash including the encapsulated phytochemicals exhibited anti-growth ac-tivity against Gram (+) and Malassezia furfur which is known to cause dandruff. In cytotoxicity test against HDF (human dermal fibroblast), we could not detect any toxicity when the supramolecules content was less than 1 mg/mL. Our results suggest that the supramolecule of β-cyclodextrin with phytochemicals could be a safe anti-bac-terial agent for cosmeceuticals.

Key Words

phytochemical, cyclodextrin, supramolecule, encapsulation, oriental hearbs

신계호 Kyeho Shin , 윤유나 Yuna Yun , 전기붕 Giboong Jeon , 이태룡 Tae Ryong Lee , 이성원 Sungwon Yi , 조준철 Jun-cheol Cho , 박지용 Jiyong Park

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Abstract

Biofilms are surface-attached microbial communities with phenotypic and biochemical properties distinct from free-living planktonic cells. Biofilm bacteria show much greater resistance than planktonic counterparts and much higher concentration of biocide is needed to treat biofilms compared to the dosage used for planktonic bacteria. As a result, alternative strategies or more effective agents exhibiting activity against biofilm-producing micro-organisms are of great interest. Therefore, we turned our attention to control of biofilm of S. aureus. The aims of this research are to investigate substances which inhibit the formation of biofilm by S. aureus and to suggest effective materials for controlling skin problems. We coated slide glasses with human placental collagen and the coverslip was incubated with test materials and bacteria. The coverslip was stained with crystal violet and we measured optical density of each sample. The biofilm inhibitory activity was calculated by crystal violet staining degrees. In this study, S. aureus ATCC 6538 was used as test organism. Our results show that both water soluble and insoluble Hinoki cypress polysaccharide strongly inhibited biofilm formation. Whereas, green tea and sunset hibiscus root extract promoted biofilm. Xylitol showed a concentration dependent effect; high concentration (3 % and 5 %) of xylitol reduced biofilm while promoted biofilm formation at a concentration of 1 %. These results support that Hinoki cypress polysaccharide and xylitol have ability to suppress biofilm formation.

Key Words

Staphylococcus aureus, biofilm, biomaterials, Hinoki cypress, xylitol

송민현 Min Hyeon Song , 배준태 Jun Tae Bae , 고현주 Hyun Ju Ko , 장용만 Yong Man Jang , 이종대 Jong Dae Lee , 이근수 Geun Soo Lee , 표형배 Hyeong Bae Pyo

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Abstract

Citrus unshiu (C. unshiu) Markovich were dried peel of mandarin orange, of which fresh fruit was one of the famous foods in Korea and Eastern Asia. In the oriental medicine, C. unshiu peel was known to have a diuretic effect and to strengthen spleen function. Recently, natural flavonoids of C. unshiu peel have been investigated. In this study, C. unshiu peel extract containing flavonoid-glycosides was cultured with Schizophyllum commune (S. commune) mycelia producing β-glu- cosidase and its biological activities were investigated. β-glucosidase of S. commune mycelia converted the flavonoid-glycosides (rutin and hesperidin) into aglycones (naringenin and hesperetin). Fermented C. unshiu peel extract compounds were analyzed by HPLC system. The photoprotective potential of fermented C. unshiu peel extract was tested in human dermal fibroblasts (HDFs) exposed to UVA. Fermented C. unshiu peel extract extract also showed notable in vitro anti-inflammatory effect on cellular systems generating cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) metabolites. Also, UVB-induced production of interleukin-1α in human HaCaT cells was reduced in a dose-dependent manner by treatment with fermented C. unshiu peel extract. These results suggest that fermented C. unshiu peel extract may mitigate the effects of photoaging in skin by reducing UV-induced adverse skin reaction.

Key Words

anti-oxidant, anti-inflammatory, Citrus unshiu peel, Schizophyllum commune

김정은 Jung Eun Kim , 박찬하 Chan Ha Park , 오대석 Dae Seok Oh , 이승연 Seung Yeon Lee , 장세훈 Se Hun Jang , 홍지연 Jee Yeon Hong , 민혜진 Hye Jin Min , 박수아 Su Ah Park , 원두현 Doo Hyun Won , 박수남 Soo Nam Park

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Abstract

In this study, the antioxidative effects and inhibitory activities on tyrosinase of Rheum undulatum (R. undulatum) L. extracts were investigated. 50 % ethanol extract, ethyl acetate and aglycone fractions of R. undulatum L. were used in experiments. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities (FSC₅₀) of R. undulatum L. extracts was lower than (+)-α-tocopherol, known as a typical antioxidant. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) of aglycone fraction on ROS generated in Fe³⁺-EDTA/H₂O₂ system using the luminol-dependent chemiluminescence assay showed the most prominent effect at a concentration of 0.265 μg/mL. The cellular protective effects of extract/fractions of R. undulatum L. on the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner (1 ~ 50 μg/mL). Especially, aglycone fraction in 10 μg/mL concentration showed the most protective effect among extracts (τ₅₀, 757.0 min). The inhibitory effects (IC₅₀, 11.20 μg/mL) on tyrosinase of aglycone fraction was much higher than arbutin (226.88 μg/mL), known as a whitening agent. These results indicate that R. undulatum L. extracts can be used as antioxidant. Particularly, aglycone fraction of R. undulatum L. showed superior antioxdative activity and high inhibitory effect on tyrosinase. Therefore, aglycone fraction of R. undulatum L. could be applicable to new functional cosmetics.

Key Words

R. undulatum L., antioxidative activity, tyrosinase, cosmetics